Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Pyridoxine

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Description

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without PyridoxinePyridoxine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of pyridoxine or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room

Trace elements and Vitamins without Agar and Amino Acids

2 at 25°C Suspend 13 gram powdered medium in 1 litre distilled water

2 at 25°C Suspend 25 gram powdered medium in 1 litre distilled water Store dry at room temperature

20mM Glacial Acetic Acid and 1mM Na2EDTA

5 Soluble starch 7

2 at 25°C Suspend 51 gram powdered medium in 1 litre distilled water

Agar and Amino acids

Component g/l Peptone 5 Yeast Extract 0

05 L-Lysine HCl 0

In Translucent K free medium ammonium phosphate (0

FM Amino acid drop-out mixture is based on the Amino acids present in FM Minimal medium

1 CFU/ml Bacterial endotoxin < 0