HL5 Medium including Glucose supplemented with vitamins and micro elements

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Description

HL5 Medium including Glucose supplemented with vitamins and micro elementsHL5 supplemented with vitamins and micro elements as present in FM and SIH Media. The addition of extra vitamins and micro elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis Component g l Peptone 14 Yeast Extract 7 Glucose 13. 5 KH2PO4 0. 5 Na2HPO4 0. 5 FM Vitamins and Micro elements 0. 01 Suspend 35. 5 gram powdered medium in 1 litre distilled water. Store

50mM TRIS base

Chemical formula C10H16N2O3S Solubility (20° C) 0

Suspend 17

Dilute 100ml TBE Buffer 10X into 900ml deionised water to make 1 litre of TBE Buffer

01 Suspend 22 gram powdered medium in 1 litre distilled water

25 K2HPO4 0

15 Glucose 18 KH2PO4 0

The hydroxyl group present at the 3 position of G-418 is phosphorylated by NPTII

Appearance White Crystalline Powder Assay (Titration) ≥99% Water Content 8

The attachment of amino-acyl transfer RNA to its acceptor site on the ribosome is inhibited

1 CFU/ml Bacterial endotoxin < 0

For propagation of of lambda phages in Escherichia coli